Protein Purification Reagents and Kits
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit is designed to isolate CD8+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD8 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.This product replaces the EasySep™ Human Whole Blood CD8 Positive Selection Kit (Catalog #18083) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human ILC2 Isolation Kit, For processing: 2 x 10^9 cells
The EasySep™ Human ILC2 Isolation Kit is designed to isolate Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic positive selection without the use of a flow sorter.First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
Isolate highly purified CD33+ and CD66b+ myeloid cells, including myeloid progenitor cells, granulocytes, and monocytes, from fresh whole blood prior to chimerism testing. Desired cells are targeted with tetrameric antibody complexes recognizing CD33, CD66b, and dextran-coated magnetic particles, and separated without the use of columns through immunomagnetic positive selection using an EasySep™ magnet. Cells of interest remain in the tube while unwanted cells are poured off. This product can be used with RoboSep™ to automate immunomagnetic cell isolation. Visit www.EasySep.com for more information.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection is designed to isolate CD4+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD4 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD4 antigen is expressed on helper T cells and more weakly on monocytes and tissue macrophages.This product replaces the EasySep™ Human Whole Blood CD4 Positive Selection Kit (Catalog #18082) for even faster cell isolations.
STEMCELL Technologies EasySep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood, For processing: 200 mL whole blood processed
The EasySep™ Total Lymphocyte Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human lymphocytes from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD14, CD15, CD16, CD33, CD36, CD41, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies RoboSep™ Human PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human PE Positive Selection Kit II is designed to isolate cells that are labeled with PE (phycoerythrin)-conjugated antibodies by positive selection. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human PE Positive Selection Kit (Catalog #18551). For highly purified particle-free cells, we recommend the EasySep™ Release Human PE Positive Selection Kit (Catalog #17654) (https://www.stemcell.com/products/easysep-release-human-pe-positive-selection-kit.html)
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ HLA Granulocyte Depletion Cocktail, For processing: 250 mL whole blood processed
Immunodensity depletion of granulocytes for HLA analysis
Genscript Corporation CD4/LEU3 His Avi Human
CD4 also known as L3T4 T4 and W3/25 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes It is a standard phenotype marker for the identification of T cell populations Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses In T-cells functions primarily as a coreceptor for MHC class II molecule peptide complex
Genscript Corporation Her2/ErbB2 His Mouse
ErbB2 also called Neu and Her2 (human epidermal growth factor receptor 2) is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors Upon ERBB2 activation the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane This prevents the phosphorylation of APC and CLASP2 allowing its association with the cell membrane
Bioss Dengue virus
Dengue virus Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Genscript Corporation CytoSinct 1000 Cell Isolation
GenScript has developed the CytoSinct 1000 Cell Isolation system for easy automated isolation or depletion of human cells Scientists can now isolate up to 120x10 9 total cells (full leukopak) in a single run within 2 hours
Genscript Corporation Her2/ErbB2 His Cynomolgus
ErbB2 also called Neu and Her2 (human epidermal growth factor receptor 2) is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors Upon ERBB2 activation the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane
Tosoh Bioscience LLC TSKGEL AMIDE-80 2X50 5UM
Column, Normal Phase/Hydrophilic Interaction Chromatography; Tosoh TSKgel Amide-80; Media: Silica; Particle: 5 micrometer; Pore Size: 80 angstrom; 2mm I.D. x 5cm Length; Stainless Steel